If a probiotic is intended to be used as a drug for the diagnosis, treatment, mitigation, cure, or prevention of disease, it must be subject to regulatory procedures as a drug. To ensure the safety of biological agents, one of the commonly used test methods is the cytotoxicity test. Cytotoxicity tests can observe the cell growth, activity, and morphological effect of the drug in vitro. Considering the advantages of cytotoxicity testing, researchers used it as a pilot test before applying it to animals. The method proves valuable in identifying the off-target effects of certain drug compounds on the human body. Learn more: Identification of Cytotoxicity In Vitro